• Login
    View Item 
    •   eScholar Home
    • Faculty of Science
    • Master Theses & Projects
    • View Item
    •   eScholar Home
    • Faculty of Science
    • Master Theses & Projects
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Forensic electrochemistry: time since deposition (TSD) estimation of degrading bloodstains using differential pulse voltammetry

    Thumbnail
    View/Open
    Tiessen_Mitchell.pdf (4.939Mb)
    Date
    2021-12-01
    Author
    Tiessen, Mitchell
    Metadata
    Show full item record
    Abstract
    Methods for bloodstain time since deposition (TSD) estimation are still in development. Hemoglobin (Hb) oxidative changes remain one of the primary means of bloodstain aging. This research explores the use of differential pulse voltammetry as a technique to analyze degrading bloodstains. The optimized conditions were determined to be 1μL of whole blood analyzed in a pH 7.0 phosphate buffer. This protocol was used to measure changes in electrochemical response in a two-week time series experiment using 9 biological replicates across 5 environmental conditions. Linear mixed models suggested that the peak height and area ratios for the Hb redox reactions were significantly correlated with time (p < 0.033). Absolute dating and principal component analysis demonstrate significant changes (p < 0.043) at the 96-hour time point and present opportunities for forensic TSD predictions. Overall, the changes in Hb redox peaks over time offers insight into oxidative changes and cellular degradation in bloodstains.
    URI
    https://hdl.handle.net/10155/1399
    Collections
    • Electronic Theses and Dissertations [1323]
    • Master Theses & Projects [292]

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV
     

     

    Browse

    All of eScholarCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV