Browsing by Author "Desaulniers, Jean-Paul"
Now showing items 1-12 of 12
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Analysis of the volatile organic compounds produced by the decomposition of pig carcasses and human remains
Stadler, Sonja (2013-03-01)Complex processes of decomposition produce a variety of chemicals as soft tissues and their component parts are degraded. Among others, these decomposition by-products include volatile organic compounds (VOCs) responsible ... -
The conjugation and evaluation of hydrophobic small molecules to trazole-linked siRNAs
Peel, Brandon (2014-08-01)According to the Human Development Index, it is estimated that all cancer cases will rise from 12.7 million cases in 2008 to 22.2 million by 2030. Traditional therapies, including surgery, radiation therapy, and chemotherapy, ... -
Development and characterization of chemical modifications for siRNA strand selection
Varley, Andrew James (2021-12-01)Small interfering RNA (siRNA) are non-coding, double-stranded RNA that enable efficient gene silencing through RNA interference (RNAi) mechanisms. The primary effector of RNAi is an RNA-guided nuclease, Ago2, which selects ... -
Investigation into potential Dirofilaria immitis drug targets through rational anthelmintic synthesis and design
Collins, Autumn M. (2022-04-01)Dirofilaria immitis is a mosquito-transmitted parasitic nematode that can cause debilitating disease in dogs. D. immitis is present in Canada and over the years has increased in prevalence due to several factors including ... -
Silence of the fish: injection of photoswitchable short interfering RNA oligonucleotides into Japanese medaka embryos (oryzias latipes) to photochemically control gene silencing
Mateus, Makenzie (2023-12-01)The siRNA duplex functions by binding to and cleaving mRNA, a process known as the RNAi pathway. Existing siRNAs face challenges such as off-target effects and unpredictable prolonged gene-silencing. To address these issues, ... -
Synthesis and in vitro assessment of chemically modified siRNAs targeting BCL2 that contain 2'-ribose and triazole-linked backbone modifications
Hagen, Gordon (2015-08-01)Short interfering RNAs (siRNAs) are biomolecules used for post-transcriptional gene regulation, and therefore hold promise as a future therapeutic by silencing gene expression of overexpressed deleterious genes. Chemically ... -
The synthesis and in vitro evaluation of chemically modified siRNAs that contain internal azobenzene derivative spacers for selective and tunable photocontrol of activity
Hammill, Matthew (2017-08-01)Short interfering RNAs (siRNAs) are biopolymers that are used as post-transcriptional gene regulators and as a natural endogenous defense against attack from viruses. These molecules hold a great deal of interest as ... -
The synthesis and in vitro evaluation of short interfering RNA containing triazole functionalized sphingolipid-based derivatives
Fernandez, Charlene A. (2021-12-01)RNA Interference (RNAi) is a natural cellular process that silences the expression of a target gene in a sequence-specific manner by mediating targeted mRNA degradation. One of the main challenges in RNAi research is ... -
The synthesis and in vitro evaluation of short interfering RNAs that contain internal modified alkyl spacers
McKim, Christopher J. (2014-08-01)The ability to silence genes effectively at the pre-translational level through the use of short interfering ribonucleic acid (siRNA) has become a widely studied area since the discovery of the RNA interference pathway in ... -
The synthesis and in vitro evaluation of siRNAs modified at the backbone with a novel triazole-based internucleotide linkage and abasic alkyl-chain linkages of varying length
Efthymiou, Tim (2013-04-01)For over a decade, the use of double-stranded short interfering RNAs (siRNAs) to silence the expression of genes associated with disease at the translational level has gained much attention. Using a highly conserved ... -
The synthesis and in vivo evaluation of chemically modified siRNAs that contain red-shifted internal azobenzene derivative spacers for selective and tunable photocontrol of activity without UV light
Hammill, Matthew (2021-08-01)Short interfering RNAs (siRNAs) are biopolymers that are used for post-transcriptional gene regulation and act as endogenous defenses against attack from viruses and dsRNA parasites. The siRNA field is making advances for ... -
Synthesis, characterization and biological investigation of self-delivering and modified short interfering RNAs (siRNAs)
Salim, Lidya (2021-07-01)Aberrant gene expression is a hallmark of disease, so it is of great interest to develop targeted therapies that provide a means to regulate gene expression. The RNA interference pathway serves as a natural defense system ...