Browsing Faculty of Science by Subject "siRNA"
Now showing items 1-8 of 8
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Development and characterization of chemical modifications for siRNA strand selection
(2021-12-01)Small interfering RNA (siRNA) are non-coding, double-stranded RNA that enable efficient gene silencing through RNA interference (RNAi) mechanisms. The primary effector of RNAi is an RNA-guided nuclease, Ago2, which selects ... -
Silence of the fish: injection of photoswitchable short interfering RNA oligonucleotides into Japanese medaka embryos (oryzias latipes) to photochemically control gene silencing
(2023-12-01)The siRNA duplex functions by binding to and cleaving mRNA, a process known as the RNAi pathway. Existing siRNAs face challenges such as off-target effects and unpredictable prolonged gene-silencing. To address these issues, ... -
Synthesis and in vitro assessment of chemically modified siRNAs targeting BCL2 that contain 2'-ribose and triazole-linked backbone modifications
(2015-08-01)Short interfering RNAs (siRNAs) are biomolecules used for post-transcriptional gene regulation, and therefore hold promise as a future therapeutic by silencing gene expression of overexpressed deleterious genes. Chemically ... -
The synthesis and in vitro evaluation of chemically modified siRNAs that contain internal azobenzene derivative spacers for selective and tunable photocontrol of activity
(2017-08-01)Short interfering RNAs (siRNAs) are biopolymers that are used as post-transcriptional gene regulators and as a natural endogenous defense against attack from viruses. These molecules hold a great deal of interest as ... -
The synthesis and in vitro evaluation of short interfering RNA containing triazole functionalized sphingolipid-based derivatives
(2021-12-01)RNA Interference (RNAi) is a natural cellular process that silences the expression of a target gene in a sequence-specific manner by mediating targeted mRNA degradation. One of the main challenges in RNAi research is ... -
The synthesis and in vitro evaluation of short interfering RNAs that contain internal modified alkyl spacers
(2014-08-01)The ability to silence genes effectively at the pre-translational level through the use of short interfering ribonucleic acid (siRNA) has become a widely studied area since the discovery of the RNA interference pathway in ... -
The synthesis and in vivo evaluation of chemically modified siRNAs that contain red-shifted internal azobenzene derivative spacers for selective and tunable photocontrol of activity without UV light
(2021-08-01)Short interfering RNAs (siRNAs) are biopolymers that are used for post-transcriptional gene regulation and act as endogenous defenses against attack from viruses and dsRNA parasites. The siRNA field is making advances for ... -
Synthesis, characterization and biological investigation of self-delivering and modified short interfering RNAs (siRNAs)
(2021-07-01)Aberrant gene expression is a hallmark of disease, so it is of great interest to develop targeted therapies that provide a means to regulate gene expression. The RNA interference pathway serves as a natural defense system ...