Search
Now showing items 1-5 of 5
The synthesis and in vitro evaluation of short interfering RNA containing triazole functionalized sphingolipid-based derivatives
(2021-12-01)
RNA Interference (RNAi) is a natural cellular process that silences the expression of a target gene in a sequence-specific manner by mediating targeted mRNA degradation. One of the main challenges in RNAi research is ...
Development and characterization of chemical modifications for siRNA strand selection
(2021-12-01)
Small interfering RNA (siRNA) are non-coding, double-stranded RNA that enable efficient gene silencing through RNA interference (RNAi) mechanisms. The primary effector of RNAi is an RNA-guided nuclease, Ago2, which selects ...
The synthesis and in vivo evaluation of chemically modified siRNAs that contain red-shifted internal azobenzene derivative spacers for selective and tunable photocontrol of activity without UV light
(2021-08-01)
Short interfering RNAs (siRNAs) are biopolymers that are used for post-transcriptional gene regulation and act as endogenous defenses against attack from viruses and dsRNA parasites. The siRNA field is making advances for ...
Synthesis, characterization and biological investigation of self-delivering and modified short interfering RNAs (siRNAs)
(2021-07-01)
Aberrant gene expression is a hallmark of disease, so it is of great interest to develop targeted therapies that provide a means to regulate gene expression. The RNA interference pathway serves as a natural defense system ...
Silence of the fish: injection of photoswitchable short interfering RNA oligonucleotides into Japanese medaka embryos (oryzias latipes) to photochemically control gene silencing
(2023-12-01)
The siRNA duplex functions by binding to and cleaving mRNA, a process known as the RNAi pathway. Existing siRNAs face challenges such as off-target effects and unpredictable prolonged gene-silencing. To address these issues, ...